Inulin coated plasmonic gold nanoparticles as a tumor-selective tool for cancer therapy.

نویسندگان

  • Anna Li Volsi
  • Dorleta Jimenez de Aberasturi
  • Malou Henriksen-Lacey
  • Gaetano Giammona
  • Mariano Licciardi
  • Luis M Liz-Marzán
چکیده

Polymer coated gold nanospheres are proposed as a tumor selective carrier for the anticancer drug doxorubicin. Thiolated polyethyleneglycol (PEG-SH) and an inulin-amino derivative based copolymer (INU-EDA) were used as stabilizing and coating materials for 40 nm gold nanospheres. The resulting polymer coated gold nanospheres (Au@PEG-INU) showed excellent physicochemical stability and potential stealth like behavior. The system was loaded with doxorubicin (Au@PEG-INU/Doxo) and its cytotoxicity profile was evaluated on human cervical cancer cells (HeLa) and lung cancer cells (A549), as compared to Au@PEG-INU and doxorubicin alone. Cytotoxicity assays showed that the system is able to drastically reduce cell viability upon incubation for 3 days. This result was supported by the ability of Au@PEG-INU/Doxo to be internalized by cancer cells and to release doxorubicin, as assessed by fluorescence microscopy. Finally, a cancer/non cancer cell co-culture model was used to display the advantageous therapeutic effects of the proposed system with respect to doxorubicin alone, thereby demonstrating the ability of Au@PEG-INU/Doxo to preferentially accumulate in tumor cells due to their enhanced metabolism, and to selectively kill target cells.

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منابع مشابه

Inulin coated plasmonic gold nanoparticles as a tumor-selective tool for cancer therapy† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c5tb01810b Click here for additional data file.

1Department of Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche (STEBICEF), University of Palermo, Italy. 2Bionanoplasmonics Laboratory, CIC BiomaGUNE, Paseo de Miramón 182, 20009 Donostia San-Sebastián, Spain. 3Mediterranean Center for Human Health Advanced Biotechnologies (Med-CHAB), Palermo, Italy. 4Ikerbasque, Basque Foundation for Science, 48013 Bilbao, Spain. 5Biomedical Research ...

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عنوان ژورنال:
  • Journal of materials chemistry. B

دوره 4 6  شماره 

صفحات  -

تاریخ انتشار 2016